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ice crispr analysis web tool  (Synthego Inc)

 
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    Structured Review

    Synthego Inc ice crispr analysis web tool
    Disruption of the SIRPA gene in the THP‐1 monocytic cell line using the <t>CRISPR/Cas9</t> technique. (A) A schematic diagram illustrates the procedure to generate the SIRPα knockout (KO) THP‐1 cells, followed by characterization and phagocytosis assay (created by BioRender.com /Mahidol University). (B) Gene editing profile of the sorted SIRPα‐negative cells (SIRPα KO THP‐1) compared to the reference sequences from the wild‐type (WT) THP‐1 cells. (C) Representative histogram shows the expression levels of SIRPα protein in the WT THP‐1 cells and sorted SIRPα‐negative THP‐1 cells (SIRPα KO THP‐1). The number in black represents the percentage of the negative population, the number in green represents the mean fluorescence intensity (MFI), and the vertical dashed line indicates the background of the isotype control. The presented data are representative of three independent biological replicates.
    Ice Crispr Analysis Web Tool, supplied by Synthego Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ice+crispr+analysis+web+tool/analysis+crispr+ice+tool/pmc12631164-210-7-12
    Average 86 stars, based on 1 article reviews
    ice crispr analysis web tool - by Bioz Stars, 2026-09
    86/100 stars

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    1) Product Images from "Enhancing macrophage phagocytosis of cancers by disrupting the SIRPα / CD47 signaling axis and targeting MUC1 antigen"

    Article Title: Enhancing macrophage phagocytosis of cancers by disrupting the SIRPα / CD47 signaling axis and targeting MUC1 antigen

    Journal: The Febs Journal

    doi: 10.1111/febs.70192

    Disruption of the SIRPA gene in the THP‐1 monocytic cell line using the CRISPR/Cas9 technique. (A) A schematic diagram illustrates the procedure to generate the SIRPα knockout (KO) THP‐1 cells, followed by characterization and phagocytosis assay (created by BioRender.com /Mahidol University). (B) Gene editing profile of the sorted SIRPα‐negative cells (SIRPα KO THP‐1) compared to the reference sequences from the wild‐type (WT) THP‐1 cells. (C) Representative histogram shows the expression levels of SIRPα protein in the WT THP‐1 cells and sorted SIRPα‐negative THP‐1 cells (SIRPα KO THP‐1). The number in black represents the percentage of the negative population, the number in green represents the mean fluorescence intensity (MFI), and the vertical dashed line indicates the background of the isotype control. The presented data are representative of three independent biological replicates.
    Figure Legend Snippet: Disruption of the SIRPA gene in the THP‐1 monocytic cell line using the CRISPR/Cas9 technique. (A) A schematic diagram illustrates the procedure to generate the SIRPα knockout (KO) THP‐1 cells, followed by characterization and phagocytosis assay (created by BioRender.com /Mahidol University). (B) Gene editing profile of the sorted SIRPα‐negative cells (SIRPα KO THP‐1) compared to the reference sequences from the wild‐type (WT) THP‐1 cells. (C) Representative histogram shows the expression levels of SIRPα protein in the WT THP‐1 cells and sorted SIRPα‐negative THP‐1 cells (SIRPα KO THP‐1). The number in black represents the percentage of the negative population, the number in green represents the mean fluorescence intensity (MFI), and the vertical dashed line indicates the background of the isotype control. The presented data are representative of three independent biological replicates.

    Techniques Used: Disruption, CRISPR, Knock-Out, Phagocytosis Assay, Expressing, Fluorescence, Control

    Related Articles

    Knock-Out:

    Article Title: Enhancing macrophage phagocytosis of cancers by disrupting the SIRPα / CD47 signaling axis and targeting MUC1 antigen
    Article Snippet: .. The knockout efficiency was determined using the ICE CRISPR analysis Web Tool (Synthego, Redwood City, CA, USA). .. Differentiation of THP‐1 cells toward M0 macrophages was performed by culturing the cells in a complete RPMI 1640 medium supplemented with 10 ng·mL −1 phorbol 12‐myristate 13‐acetate (PMA, Peprotech) for 24 h. The following day, the culture medium was replaced with a complete RPMI 1640 medium without PMA supplementation, and the cells were incubated for an additional 3 days.

    Article Title: Enhancing macrophage phagocytosis of cancers by disrupting the SIRPα/CD47 signaling axis and targeting MUC1 antigen.
    Article Snippet: .. The knockout efficiency was determined using the ICE CRISPR analysis Web Tool (Synthego, Redwood City, CA, USA). .. Differentiation of THP-1 cells toward M0 macrophages was performed by culturing the cells in a complete RPMI 1640 medium supplemented with 10 ng mL 1 phorbol 12-myristate 13-acetate (PMA, Peprotech) for 24 h. The following day, the culture medium was replaced with a complete RPMI 1640 medium without PMA supplementation, and the cells were incubated for an additional 3 days.

    CRISPR:

    Article Title: Enhancing macrophage phagocytosis of cancers by disrupting the SIRPα / CD47 signaling axis and targeting MUC1 antigen
    Article Snippet: .. The knockout efficiency was determined using the ICE CRISPR analysis Web Tool (Synthego, Redwood City, CA, USA). .. Differentiation of THP‐1 cells toward M0 macrophages was performed by culturing the cells in a complete RPMI 1640 medium supplemented with 10 ng·mL −1 phorbol 12‐myristate 13‐acetate (PMA, Peprotech) for 24 h. The following day, the culture medium was replaced with a complete RPMI 1640 medium without PMA supplementation, and the cells were incubated for an additional 3 days.

    Article Title: Enhancing macrophage phagocytosis of cancers by disrupting the SIRPα/CD47 signaling axis and targeting MUC1 antigen.
    Article Snippet: .. The knockout efficiency was determined using the ICE CRISPR analysis Web Tool (Synthego, Redwood City, CA, USA). .. Differentiation of THP-1 cells toward M0 macrophages was performed by culturing the cells in a complete RPMI 1640 medium supplemented with 10 ng mL 1 phorbol 12-myristate 13-acetate (PMA, Peprotech) for 24 h. The following day, the culture medium was replaced with a complete RPMI 1640 medium without PMA supplementation, and the cells were incubated for an additional 3 days.



    Similar Products

    ice crispr analysis web tool  (Synthego Inc)
    86
    Synthego Inc ice crispr analysis web tool
    Disruption of the SIRPA gene in the THP‐1 monocytic cell line using the <t>CRISPR/Cas9</t> technique. (A) A schematic diagram illustrates the procedure to generate the SIRPα knockout (KO) THP‐1 cells, followed by characterization and phagocytosis assay (created by BioRender.com /Mahidol University). (B) Gene editing profile of the sorted SIRPα‐negative cells (SIRPα KO THP‐1) compared to the reference sequences from the wild‐type (WT) THP‐1 cells. (C) Representative histogram shows the expression levels of SIRPα protein in the WT THP‐1 cells and sorted SIRPα‐negative THP‐1 cells (SIRPα KO THP‐1). The number in black represents the percentage of the negative population, the number in green represents the mean fluorescence intensity (MFI), and the vertical dashed line indicates the background of the isotype control. The presented data are representative of three independent biological replicates.
    Ice Crispr Analysis Web Tool, supplied by Synthego Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ice+crispr+analysis+web+tool/analysis+crispr+ice+tool/pmc12631164-210-7-12
    Average 86 stars, based on 1 article reviews
    ice crispr analysis web tool - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    Disruption of the SIRPA gene in the THP‐1 monocytic cell line using the CRISPR/Cas9 technique. (A) A schematic diagram illustrates the procedure to generate the SIRPα knockout (KO) THP‐1 cells, followed by characterization and phagocytosis assay (created by BioRender.com /Mahidol University). (B) Gene editing profile of the sorted SIRPα‐negative cells (SIRPα KO THP‐1) compared to the reference sequences from the wild‐type (WT) THP‐1 cells. (C) Representative histogram shows the expression levels of SIRPα protein in the WT THP‐1 cells and sorted SIRPα‐negative THP‐1 cells (SIRPα KO THP‐1). The number in black represents the percentage of the negative population, the number in green represents the mean fluorescence intensity (MFI), and the vertical dashed line indicates the background of the isotype control. The presented data are representative of three independent biological replicates.

    Journal: The Febs Journal

    Article Title: Enhancing macrophage phagocytosis of cancers by disrupting the SIRPα / CD47 signaling axis and targeting MUC1 antigen

    doi: 10.1111/febs.70192

    Figure Lengend Snippet: Disruption of the SIRPA gene in the THP‐1 monocytic cell line using the CRISPR/Cas9 technique. (A) A schematic diagram illustrates the procedure to generate the SIRPα knockout (KO) THP‐1 cells, followed by characterization and phagocytosis assay (created by BioRender.com /Mahidol University). (B) Gene editing profile of the sorted SIRPα‐negative cells (SIRPα KO THP‐1) compared to the reference sequences from the wild‐type (WT) THP‐1 cells. (C) Representative histogram shows the expression levels of SIRPα protein in the WT THP‐1 cells and sorted SIRPα‐negative THP‐1 cells (SIRPα KO THP‐1). The number in black represents the percentage of the negative population, the number in green represents the mean fluorescence intensity (MFI), and the vertical dashed line indicates the background of the isotype control. The presented data are representative of three independent biological replicates.

    Article Snippet: The knockout efficiency was determined using the ICE CRISPR analysis Web Tool (Synthego, Redwood City, CA, USA).

    Techniques: Disruption, CRISPR, Knock-Out, Phagocytosis Assay, Expressing, Fluorescence, Control